Plasmid_Backbone

Part:BBa_K572200:Design

Designed by: Chaitra Prabhakara   Group: iGEM11_IIT_Madras   (2011-10-05)


Light based expression vector- pSB1Pe


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found at 130
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2399
    Illegal SpeI site found at 131
    Illegal PstI site found at 145
    Illegal NotI site found at 138
    Illegal NotI site found at 2405
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2399
    Illegal XhoI site found at 1162
    Illegal XhoI site found at 2144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2399
    Illegal suffix found at 131
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2399
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2414
    Illegal SpeI site found at 131
    Illegal PstI site found at 145
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Design Notes

The reading frame should be considered while designing


Source

Plasmid Backbone - Psb1c3 Parts - B0015, K118011

References