Part:BBa_K572004
PcstA_Luciferase Generator
The part was designed to use it as a control in our experiments with proteorhodopsin. RFP production is estimated using luciferase activity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 684
The biobrick in pSB1A2 was transformed into DH5alpha cells. This primary culture was used as an inoculum for the secondary culture. After the OD reached 0.6, the cells were resuspended in M9 Media with different glucose concentrations - 0, 0.02, 0.1, 0.5, LB and LB+0.2.
The biobrick contains a eukaryotic ribosome site. This experiment serves as a characterization of the ribosome binding site. Luciferase Assay showed basal level expression of the enzyme even after 6 hours after introducing cells into M9 media. The eukaryotic ribosome binding site didn't result in expression of the protein following it in E.coli.
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