Coding

Part:BBa_K569017:Design

Designed by: David Golynskiy and Tyler Guinn   Group: iGEM11_UT_Dallas   (2011-09-27)

CheY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 275
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 275
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 275
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 275
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used primers: ccatctagacacctgcaaaaggagatgg and ccaactagtcacctgcaaaaagggacat to PCR CheY from K422007 from ETHZ_Basel 2010. We added in the correct BioBrick restriction enzymes in the PCR and placed it into the pSB1C3 backbone.

Source

K422007 ETHZ_Basel 2010

References