Part:BBa_K567022

PT7-Luc-2AGG-tRNA(Arg)

This part is constructed based on PT7-Luc-2AGG (BBa_K567008). The tDNA^Arg and luciferase gene into T7 operon so that both genes can be transcribed. tRNA^Arg can be correctly processed and become matured. Luciferase mRNA will be further translated into protein. The induced tRNA^Arg can facilitate the translation of luciferase-2AGG, achieving positive feedback. The transcription of this part is under the control of T7 promoter and lac operator. RNA expression is induced by 0.5mM IPTG when the OD600 of the culture reaches 0.3.

The working curve is shown here.

Related Biobrick

PT7-Luc-2AGG-tRNA(Arg) (BBa_K567022)

PT7-Luc-4AGG-tRNA(Arg) (BBa_K567023)

PT7-Luc-6AGG-tRNA(Arg) (BBa_K567024)

PT7-Luc-8AGG-tRNA(Arg) (BBa_K567025)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 48
  Illegal PstI site found at 1923
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 1923
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 48
  Illegal PstI site found at 1923
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 48
  Illegal PstI site found at 1923
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 911