Composite
Part:BBa_K566042:Design
Designed by: Porfirio Quintero-Cadena, José Eduardo Almeyda Carbajal Group: iGEM11_UANL_Mty-Mexico (2011-09-28)
composite Biphasic switch
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2454
Illegal BglII site found at 2510
Illegal BglII site found at 2684
Illegal XhoI site found at 2548 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 547
Illegal AgeI site found at 558
Illegal AgeI site found at 1959 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1450
Design Notes
Search for illegal sites and it was optimized for E.coli codon usage
Source
Planning