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Composite

Part:BBa_K566041:Design

Designed by: José Eduardo Almeyda Carbajal   Group: iGEM11_UANL_Mty-Mexico   (2011-09-28)


Second State for Biphasic switch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1586
    Illegal BglII site found at 1642
    Illegal BglII site found at 1816
    Illegal XhoI site found at 1680
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 547
    Illegal AgeI site found at 558
    Illegal AgeI site found at 1091
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites was performed and it was optimized for E. coli codon usage


Source

Planning

References