Generator

Part:BBa_K566030:Design

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-27)

CcaS gene (constitutive expression)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1299
    Illegal SpeI site found at 1394
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1299
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 670
    Illegal SpeI site found at 1394
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1299
    Illegal XhoI site found at 2316
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1299
    Illegal SpeI site found at 1394
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1299
    Illegal SpeI site found at 1394
    Illegal AgeI site found at 1334
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Due to illegal sites it was implemented UANLBricks assambly



Source

UANLBricks assambly

References

1. Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in Escherichia coli. J Mol Biol 405:315-324.