Generator

Part:BBa_K566029:Design

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-27)

CcaR gene (constitutive expression)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 69
    Illegal SpeI site found at 765
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 69
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 765
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 69
    Illegal BamHI site found at 466
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 69
    Illegal SpeI site found at 765
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 69
    Illegal SpeI site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

UANLBrick assambly


Source

UANLBrick assambly

References

1. Tabor JJ, Levskaya A, Voigt CA (2010) Multichromatic Control of Gene Expression in Escherichia coli. J Mol Biol 405:315-324.