Coding
PenI inv

Part:BBa_K566010:Design

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)

PenI repressor optimized for E. coli (inverted sequence)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Search for illegal sites was performed, they were found and eliminated.


Source

Original sequence was taken from Registry (BBa_C0074), this was optimized with preferential codons for better expression in E. coli.

References

1. Wittman V, Lin HC, Wong HC (1993) Functional domains of the penicillinase repressor of Bacillus licheniformis. J Bacteriol 175:7383-7390