Reporter
CFP opt

Part:BBa_K566003:Design

Designed by: Daniel Rodriguez   Group: iGEM11_UANL_Mty-Mexico   (2011-09-26)

CFP optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 28
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Preferential codons were implemented for improving expression in E. coli.

Analysis of illegal sites was made, they were found and removed.


Source

Synthesized DNA.

References

1. Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. Cell Science at a Glance 5:837-838