Part:BBa_K565001

Colicin G gene cluster

Colicin G is a protein produced by Escherichia coli CA46 strain. They make holes in bacterial membranes, being lethal for related strains of E. coli.

The Colicins G proteins have specific activity, they need the presence of Fiu protein in the outer membrane of target cells in order to recognize them, and use the TonB/ExbB, D translocation route to slip in the cell and lyse it.

In our project, we used Colicin G to kill pathogen cells (in our case they were E. coli DH5alpha strain) by membrane lysis, disinfecting the contaminated water.

We got this part from UK's Health Protection Agency strain collection.

Bibliography

Cascales E. et al. Colicin Biology. MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, Mar. 2007, p. 158–229 Vol. 71, No. 1.

Patzer S. I. et al. The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN. Microbiology (2003), 149, 2557–2570.

Bradley D. E. 1991. Colicins G and H and their host strains. Can. J. Microbiol. 37: 751-757.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 906
  Illegal EcoRI site found at 3673
  Illegal EcoRI site found at 4423
  Illegal EcoRI site found at 4968
  Illegal EcoRI site found at 7426
  Illegal EcoRI site found at 9490
  Illegal XbaI site found at 6611
  Illegal PstI site found at 1611
  Illegal PstI site found at 5169
  Illegal PstI site found at 5178
  Illegal PstI site found at 5720
  Illegal PstI site found at 8418
  Illegal PstI site found at 8458
  Illegal PstI site found at 8631
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 906
  Illegal EcoRI site found at 3673
  Illegal EcoRI site found at 4423
  Illegal EcoRI site found at 4968
  Illegal EcoRI site found at 7426
  Illegal EcoRI site found at 9490
  Illegal PstI site found at 1611
  Illegal PstI site found at 5169
  Illegal PstI site found at 5178
  Illegal PstI site found at 5720
  Illegal PstI site found at 8418
  Illegal PstI site found at 8458
  Illegal PstI site found at 8631
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 906
  Illegal EcoRI site found at 3673
  Illegal EcoRI site found at 4423
  Illegal EcoRI site found at 4968
  Illegal EcoRI site found at 7426
  Illegal EcoRI site found at 9490
  Illegal BglII site found at 3519
  Illegal BglII site found at 6011
  Illegal BglII site found at 6174
  Illegal BamHI site found at 7419
  Illegal BamHI site found at 7472
  Illegal BamHI site found at 8045
  Illegal BamHI site found at 10157
  Illegal BamHI site found at 11618
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 906
  Illegal EcoRI site found at 3673
  Illegal EcoRI site found at 4423
  Illegal EcoRI site found at 4968
  Illegal EcoRI site found at 7426
  Illegal EcoRI site found at 9490
  Illegal XbaI site found at 6611
  Illegal PstI site found at 1611
  Illegal PstI site found at 5169
  Illegal PstI site found at 5178
  Illegal PstI site found at 5720
  Illegal PstI site found at 8418
  Illegal PstI site found at 8458
  Illegal PstI site found at 8631
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 906
  Illegal EcoRI site found at 3673
  Illegal EcoRI site found at 4423
  Illegal EcoRI site found at 4968
  Illegal EcoRI site found at 7426
  Illegal EcoRI site found at 9490
  Illegal XbaI site found at 6611
  Illegal PstI site found at 1611
  Illegal PstI site found at 5169
  Illegal PstI site found at 5178
  Illegal PstI site found at 5720
  Illegal PstI site found at 8418
  Illegal PstI site found at 8458
  Illegal PstI site found at 8631
  Illegal AgeI site found at 102
  Illegal AgeI site found at 1081
  Illegal AgeI site found at 10538
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 9124
  Illegal SapI site found at 10233
  Illegal SapI.rc site found at 6058
  Illegal SapI.rc site found at 6978