Coding

Part:BBa_K563051:Design

Designed by: Guo Jing   Group: iGEM11_Tianjin   (2011-10-05)


pSBY11 linked with Tor2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4783
    Illegal EcoRI site found at 5203
    Illegal EcoRI site found at 5486
    Illegal EcoRI site found at 6362
    Illegal XbaI site found at 2991
    Illegal XbaI site found at 3936
    Illegal XbaI site found at 5743
    Illegal XbaI site found at 6358
    Illegal XbaI site found at 7188
    Illegal XbaI site found at 9208
    Illegal SpeI site found at 7140
    Illegal PstI site found at 8245
    Illegal PstI site found at 9991
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4783
    Illegal EcoRI site found at 5203
    Illegal EcoRI site found at 5486
    Illegal EcoRI site found at 6362
    Illegal NheI site found at 5583
    Illegal NheI site found at 7500
    Illegal SpeI site found at 7140
    Illegal PstI site found at 8245
    Illegal PstI site found at 9991
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4783
    Illegal EcoRI site found at 5203
    Illegal EcoRI site found at 5486
    Illegal EcoRI site found at 6362
    Illegal BglII site found at 2154
    Illegal BglII site found at 7422
    Illegal BamHI site found at 5769
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4783
    Illegal EcoRI site found at 5203
    Illegal EcoRI site found at 5486
    Illegal EcoRI site found at 6362
    Illegal XbaI site found at 2991
    Illegal XbaI site found at 3936
    Illegal XbaI site found at 5743
    Illegal XbaI site found at 6358
    Illegal XbaI site found at 7188
    Illegal XbaI site found at 9208
    Illegal SpeI site found at 7140
    Illegal PstI site found at 8245
    Illegal PstI site found at 9991
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4783
    Illegal EcoRI site found at 5203
    Illegal EcoRI site found at 5486
    Illegal EcoRI site found at 6362
    Illegal XbaI site found at 2991
    Illegal XbaI site found at 3936
    Illegal XbaI site found at 5743
    Illegal XbaI site found at 6358
    Illegal XbaI site found at 7188
    Illegal XbaI site found at 9208
    Illegal SpeI site found at 7140
    Illegal PstI site found at 8245
    Illegal PstI site found at 9991
    Illegal NgoMIV site found at 5122
    Illegal AgeI site found at 4362
    Illegal AgeI site found at 7519
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1601
    Illegal BsaI.rc site found at 11072
    Illegal SapI.rc site found at 4792
    Illegal SapI.rc site found at 5044
    Illegal SapI.rc site found at 9813


Design Notes

In order to conduct the mutation easily, we separate Tor2 into three parts. And then we reconstruct pSB1A2 by adding specific designed restriction sites for linking Tor2.


Source

pSB1A2 is from BioBrick.

References