Coding

Part:BBa_K563033:Design

Designed by: Guo Jing   Group: iGEM11_Tianjin   (2011-10-05)


pEASY with third part of Tor2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 910
    Illegal XbaI site found at 906
    Illegal XbaI site found at 1736
    Illegal XbaI site found at 2843
    Illegal SpeI site found at 258
    Illegal SpeI site found at 1688
    Illegal PstI site found at 2803
    Illegal PstI site found at 3993
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 910
    Illegal NheI site found at 2048
    Illegal SpeI site found at 258
    Illegal SpeI site found at 1688
    Illegal PstI site found at 2803
    Illegal PstI site found at 3993
    Illegal NotI site found at 2824
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 910
    Illegal BglII site found at 1970
    Illegal BglII site found at 3780
    Illegal BamHI site found at 252
    Illegal BamHI site found at 317
    Illegal XhoI site found at 2831
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 910
    Illegal XbaI site found at 906
    Illegal XbaI site found at 1736
    Illegal XbaI site found at 2843
    Illegal SpeI site found at 258
    Illegal SpeI site found at 1688
    Illegal PstI site found at 2803
    Illegal PstI site found at 3993
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 910
    Illegal XbaI site found at 906
    Illegal XbaI site found at 1736
    Illegal XbaI site found at 2843
    Illegal SpeI site found at 258
    Illegal SpeI site found at 1688
    Illegal PstI site found at 2803
    Illegal PstI site found at 3993
    Illegal NgoMIV site found at 3169
    Illegal NgoMIV site found at 4444
    Illegal AgeI site found at 2067
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5343
    Illegal SapI.rc site found at 4293
    Illegal SapI.rc site found at 4503


Design Notes

We separate Tor2 into three parts, and the third part is called 33Tor2. pEASY is a blunt vector provided by Transgene Company. We link 33Tor2 with this vector for further connection.What's more, the second part and the third part have some overlap for future work.


Source

33Tor2 is from genomic sequence and vector is bought from Transgene Company

References