Coding

Part:BBa_K563012:Design

Designed by: Boxuan Zeng   Group: iGEM11_Tianjin   (2011-10-05)


Tor2 with I1957T mutation


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal XbaI site found at 2865
    Illegal XbaI site found at 3810
    Illegal XbaI site found at 5617
    Illegal XbaI site found at 6232
    Illegal XbaI site found at 7062
    Illegal SpeI site found at 7014
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal NheI site found at 5457
    Illegal NheI site found at 7374
    Illegal SpeI site found at 7014
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal BglII site found at 2028
    Illegal BglII site found at 7296
    Illegal BamHI site found at 5643
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal XbaI site found at 2865
    Illegal XbaI site found at 3810
    Illegal XbaI site found at 5617
    Illegal XbaI site found at 6232
    Illegal XbaI site found at 7062
    Illegal SpeI site found at 7014
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4657
    Illegal EcoRI site found at 5077
    Illegal EcoRI site found at 5360
    Illegal EcoRI site found at 6236
    Illegal XbaI site found at 2865
    Illegal XbaI site found at 3810
    Illegal XbaI site found at 5617
    Illegal XbaI site found at 6232
    Illegal XbaI site found at 7062
    Illegal SpeI site found at 7014
    Illegal NgoMIV site found at 4996
    Illegal AgeI site found at 4236
    Illegal AgeI site found at 7393
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1475
    Illegal SapI.rc site found at 4666
    Illegal SapI.rc site found at 4918


Design Notes

We need a Tor2 with high activity to resist multi-inhibitor. This mutation help tor2 to form TORC1 with KOG1, Lst8, Tco89.


Source

We mutated BBa_K563010 to get this part.

References