Regulatory
Part:BBa_K563004:Design
Designed by: Boxuan Zeng Group: iGEM11_Tianjin (2011-10-05)
pTEF1, promoter of Translational elongation factor EF-1 alpha
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 205
Design Notes
We add NcoI and XhoI at the ends of the promoter
Source
Amplify from genome of S.cerevisiae by PCR