Regulatory

Part:BBa_K563004:Design

Designed by: Boxuan Zeng   Group: iGEM11_Tianjin   (2011-10-05)


pTEF1, promoter of Translational elongation factor EF-1 alpha


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 205


Design Notes

We add NcoI and XhoI at the ends of the promoter


Source

Amplify from genome of S.cerevisiae by PCR

References