Regulatory

Part:BBa_K563002:Design

Designed by: Boxuan Zeng   Group: iGEM11_Tianjin   (2011-10-05)


promoter of ILV2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 376
    Illegal SpeI site found at 395
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 376
    Illegal SpeI site found at 395
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 376
    Illegal BglII site found at 319
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 376
    Illegal SpeI site found at 395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 376
    Illegal SpeI site found at 395
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design NcoI and XhoI at two ends of the promoter


Source

Amplified from the genome of S.cerevisiae by PCR

References