Part:BBa_K562001

Salty_PduD20 Targeting Tag

This is a composite part comprising a constituive promoter (identical to that from the basic part BBa_K562000), which is the tatABCD promoter from E. coli K-12, driving production of the first 20 amino acid residues of the PduD protein from Salmonella enterica serovar Typhimurium LT2. This is a targeting sequence for the Salmonella Pdu bacterial microcompartment (BMC). This 20 residue targeting sequence has been tested by fusion to GFP and it does work. The construct is coloned as an EcoRI / PstI fragment into pSB1C3. The clone is also known as pSB-D20 in the Sargent Laboratory, Dundee, UK.

This part has since been improved by Manchester iGEM 2017 to https://parts.igem.org/Part:BBa_K2213006, https://parts.igem.org/Part:BBa_K2213007, and https://parts.igem.org/Part:BBa_K2213008.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 179
  
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 179
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 179
  
• 1000
  COMPATIBLE WITH RFC[1000]