Plasmid_Backbone

Part:BBa_K560016:Design

Designed by: Zhang Chao   Group: iGEM11_WHU-China   (2011-10-26)

BsaI Golden Gate Assembly_Backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 644
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 35
    Illegal NheI site found at 2776
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XhoI site found at 363
    Illegal XhoI site found at 1797
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 644
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal XbaI site found at 644
    Illegal NgoMIV site found at 1650
    Illegal AgeI site found at 569
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 575
    Illegal BsaI.rc site found at 128
    Illegal SapI.rc site found at 2653


Design Notes

N


Source

Efficient design and assembly of custom TALEN and other TAL effector-based constructs for DNA targeting. Nucleic Acids Research, 2011, Vol. 39, No. 12.

Details about the structure of this plasmid can be found in http://www.addgene.org/31021/.


References