Part:BBa_K559000:Experience
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Characterizations of BBa_K559000
Size Confirmation of the Biobrick
The above figure shows the size check of our biobrick. Lane 1 represents 100 bp DNA ladder (NEB, cat. N3231L) , lane 3 represents our biobrick halorhodopsin gene. It matched the theoretical size of 640bp.
Applications of BBa_K559000
We have used this biobrick as a basic unit to construct a functional unit BBa_K559010.
More characterizations on the regulated protein expression, chloride absorption condition and optimization were shown in BBa_K559010.
User Reviews
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BBa_K559000, integrated into the halorhodopsin complete system generator BBa_K559010, express Maxmized halorhodopsin membrane protein when supplemented with 0.1mM IPTG. Characterization of the IPTG addition to get maximum expression can be view in [1] |
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