Not Released
Sample In stock
Experience: Works
Not Used
Get This Part
Generator
BTE genera

Part:BBa_K558007:Design

Designed by: Megan Tabor   Group: iGEM11_Nevada   (2011-09-20)

Bay Laurel Thioesterse Generator for Fatty Acid Production


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 503
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 360
    Illegal BsaI.rc site found at 770


Design Notes

Codon-optimization was done on the Bay Laurel Thioesterase gene to increase the expression in E. coli. Codons were also changed to rid the sequence of illegal restriction sites. Classic cloning was done to ligate sigma 70 constitutive promoter to Bay Laurel Thioesterase for fatty acid expression.

Source

Umbellularia californica

References

T.A. Voelker, T.R. Hayes, A.C. Cranmer, H.M. Davies [1996] Plant J 9: 229–241 Welch et. al. “Design Parameters to Control Synthetic Gene Expression in Escherichia coli”