Intermediate
BTE

Part:BBa_K558003:Design

Designed by: Megan Tabor   Group: iGEM11_Nevada   (2011-09-07)

Bay Laurel Thioesterase (RBS Terminator)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 462
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 319
    Illegal BsaI.rc site found at 729


Design Notes

Codon optimizing was done to the sequence to make it compatible with E. coli.


Source

Umbellularia californica

References

. T.A. Voelker, T.R. Hayes, A.C. Cranmer, H.M. Davies [1,br996] Plant J 9: 229–241 Welch et. al. “Design Parameters to Control Synthetic Gene Expression in Escherichia coli” Welch et. al. “Design Parameters to Control Synthetic Gene Expression in Escherichia coli”