Plasmid

Part:BBa_K5535000

Designed by: Valerie Wu   Group: iGEM24_DBHS-CA   (2024-10-02)


EHMT2.V5,PuroR,GFP3.3

This plasmid is used for the study of addiction. This was assembled through Gibson Assembly to contain a CMV promoter which increases the production of EHMT2, generally known to be associated with addiction, cancer, and stress.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 3020
    Illegal XbaI site found at 3195
    Illegal XbaI site found at 8408
    Illegal SpeI site found at 3201
    Illegal SpeI site found at 7749
    Illegal PstI site found at 1054
    Illegal PstI site found at 1362
    Illegal PstI site found at 1488
    Illegal PstI site found at 1750
    Illegal PstI site found at 2286
    Illegal PstI site found at 2538
    Illegal PstI site found at 8530
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 3201
    Illegal SpeI site found at 7749
    Illegal PstI site found at 1054
    Illegal PstI site found at 1362
    Illegal PstI site found at 1488
    Illegal PstI site found at 1750
    Illegal PstI site found at 2286
    Illegal PstI site found at 2538
    Illegal PstI site found at 8530
    Illegal NotI site found at 8541
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 41
    Illegal BglII site found at 1471
    Illegal BglII site found at 3525
    Illegal BglII site found at 7512
    Illegal BamHI site found at 8827
    Illegal XhoI site found at 3014
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 3020
    Illegal XbaI site found at 3195
    Illegal XbaI site found at 8408
    Illegal SpeI site found at 3201
    Illegal SpeI site found at 7749
    Illegal PstI site found at 1054
    Illegal PstI site found at 1362
    Illegal PstI site found at 1488
    Illegal PstI site found at 1750
    Illegal PstI site found at 2286
    Illegal PstI site found at 2538
    Illegal PstI site found at 8530
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 3020
    Illegal XbaI site found at 3195
    Illegal XbaI site found at 8408
    Illegal SpeI site found at 3201
    Illegal SpeI site found at 7749
    Illegal PstI site found at 1054
    Illegal PstI site found at 1362
    Illegal PstI site found at 1488
    Illegal PstI site found at 1750
    Illegal PstI site found at 2286
    Illegal PstI site found at 2538
    Illegal PstI site found at 8530
    Illegal NgoMIV site found at 388
    Illegal NgoMIV site found at 1047
    Illegal NgoMIV site found at 3685
    Illegal NgoMIV site found at 5116
    Illegal AgeI site found at 3087
    Illegal AgeI site found at 3230
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3069
    Illegal BsaI.rc site found at 774
    Illegal BsaI.rc site found at 819
    Illegal BsaI.rc site found at 3408
    Illegal BsaI.rc site found at 6644
    Illegal SapI site found at 5561


[edit]
Categories
Parameters
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