Plasmid
Part:BBa_K5535000
Designed by: Valerie Wu Group: iGEM24_DBHS-CA (2024-10-02)
EHMT2.V5,PuroR,GFP3.3
This plasmid is used for the study of addiction. This was assembled through Gibson Assembly to contain a CMV promoter which increases the production of EHMT2, generally known to be associated with addiction, cancer, and stress.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 3020
Illegal XbaI site found at 3195
Illegal XbaI site found at 8408
Illegal SpeI site found at 3201
Illegal SpeI site found at 7749
Illegal PstI site found at 1054
Illegal PstI site found at 1362
Illegal PstI site found at 1488
Illegal PstI site found at 1750
Illegal PstI site found at 2286
Illegal PstI site found at 2538
Illegal PstI site found at 8530 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 3201
Illegal SpeI site found at 7749
Illegal PstI site found at 1054
Illegal PstI site found at 1362
Illegal PstI site found at 1488
Illegal PstI site found at 1750
Illegal PstI site found at 2286
Illegal PstI site found at 2538
Illegal PstI site found at 8530
Illegal NotI site found at 8541 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 41
Illegal BglII site found at 1471
Illegal BglII site found at 3525
Illegal BglII site found at 7512
Illegal BamHI site found at 8827
Illegal XhoI site found at 3014 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 3020
Illegal XbaI site found at 3195
Illegal XbaI site found at 8408
Illegal SpeI site found at 3201
Illegal SpeI site found at 7749
Illegal PstI site found at 1054
Illegal PstI site found at 1362
Illegal PstI site found at 1488
Illegal PstI site found at 1750
Illegal PstI site found at 2286
Illegal PstI site found at 2538
Illegal PstI site found at 8530 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 3020
Illegal XbaI site found at 3195
Illegal XbaI site found at 8408
Illegal SpeI site found at 3201
Illegal SpeI site found at 7749
Illegal PstI site found at 1054
Illegal PstI site found at 1362
Illegal PstI site found at 1488
Illegal PstI site found at 1750
Illegal PstI site found at 2286
Illegal PstI site found at 2538
Illegal PstI site found at 8530
Illegal NgoMIV site found at 388
Illegal NgoMIV site found at 1047
Illegal NgoMIV site found at 3685
Illegal NgoMIV site found at 5116
Illegal AgeI site found at 3087
Illegal AgeI site found at 3230 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3069
Illegal BsaI.rc site found at 774
Illegal BsaI.rc site found at 819
Illegal BsaI.rc site found at 3408
Illegal BsaI.rc site found at 6644
Illegal SapI site found at 5561
[edit]
Categories
Parameters
None |