Regulatory
Part:BBa_K553022:Design
Designed by: Luca Braga, Niels Ntamati Group: iGEM11_UNITS_Trieste (2011-09-17)
Eukaryotic minimal CMV promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 7
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Cloned down-stream an inducible elements and upstream a reporter gene.
Source
DNA synthesis.
References
1) Neddermann P, Gargioli C, Muraglia E, Sambucini S, Bonelli F, De Francesco R, Cortese R. A novel, inducible, eukaryotic gene expression system based on the quorum-sensing transcription factor TraR. EMBO Rep. 2003 Feb;4(2):159-65.
2) Deepak Chandran, Frank T Bergmann and Herbert M Sauro. "TinkerCell: modular CAD tool for synthetic biology". Journal of Biological Engineering 2009, 3:19 DOI: 10.1186/1754-1611-3-1