Composite

Part:BBa_K552002:Design

Designed by: Jonathan Herrmann   Group: iGEM11_WashU   (2011-09-27)


CrtYB Gene-Codon Optimized for Yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1239
    Illegal XbaI site found at 1305
    Illegal XbaI site found at 1545
    Illegal XbaI site found at 1917
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 723
    Illegal BglII site found at 1308
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1239
    Illegal XbaI site found at 1305
    Illegal XbaI site found at 1545
    Illegal XbaI site found at 1917
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1239
    Illegal XbaI site found at 1305
    Illegal XbaI site found at 1545
    Illegal XbaI site found at 1917
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.


Source

The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm

References