Composite

Part:BBa_K552001:Design

Designed by: Jonathan Herrmann   Group: iGEM11_WashU   (2011-09-24)


CrtE Gene-Codon Optimized for Yeast


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
    Illegal XbaI site found at 1229
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
    Illegal BglII site found at 491
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
    Illegal XbaI site found at 1229
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 417
    Illegal EcoRI site found at 1394
    Illegal XbaI site found at 1229
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 727


Design Notes

While designing the sequence, we made sure that the cut sites for restriction enzymes avr2 and spe1 did not occur anywhere within the sequence.


Source

The sequence of this part was originally taken from the genome of Xanthophyllomyces dendrorhous. This sequence can be found in the NCBI database. We then codon optimized this sequence for Saccharomyces cerevisiae using an online tool found here: http://www.encorbio.com/protocols/Codon.htm

References