Composite

Part:BBa_K5520012:Design

Designed by: Ruyi Shi   Group: iGEM24_Ulink-SZ   (2024-09-29)


pT7-LacO-His- CsnBV186Y


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 844
    Illegal NotI site found at 804
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 813
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 153
    Illegal AgeI site found at 636
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The objective of the study was to elucidate the structural-functional relationship of chitosanase and to facilitate the production of chitooligosaccharides with a precise degree of polymerization. To this end, the amino acid valine at position 186 was substituted with tyrosine to enhance enzymatic activity and to produce more homogeneous products.


Source

Escherichia coli and Bacillus pumilus

References