Composite

Part:BBa_K5520004:Design

Designed by: Ruyi Shi   Group: iGEM24_Ulink-SZ   (2024-09-29)


pT7-LacO-His-CDA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 149
    Illegal NheI site found at 897
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 182
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 641


Design Notes

We believe that the first step in the generation of chitosan from chitin, deacetylation of chitin to obtain chitosan, is very important. The deacetylase encoded by CDA can catalyze the deacetylation of chitin to synthesize chitosan. Therefore, we cloned the CDA gene into PET28a vector to obtain PET-28a-CDA recombinant plasmid, which was transformed into E. coli BL21 (DE3) to realize the overexpression of CDA.


Source

Escherichia coli and Bacillus pumilus

References