Coding

Part:BBa_K5520003:Design

Designed by: Ruyi Shi   Group: iGEM24_Ulink-SZ   (2024-09-29)

6×His-CsnB



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 709
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 58
    Illegal AgeI site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]



Design Notes

In the context of conducting an experiment to express and purify CsnB proteins using the pET-28a expression system, the decision to incorporate a 6×His tag was made with the purpose of simplifying the purification process and enabling protein characterization.



Source

Escherichia coli and Bacillus pumilus


References