Coding

Part:BBa_K5520002:Design

Designed by: Ruyi Shi   Group: iGEM24_Ulink-SZ   (2024-09-29)

6*His-CDA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 544


Design Notes

In designing an experiment to express and purify CDA protein by the PET-28a system, the inclusion of a 6×His tag was intended to facilitate subsequent protein purification and identification.


Source

Escherichia coli and Bacillus pumilus

References