Coding

Part:BBa_K5520002

Designed by: Ruyi Shi   Group: iGEM24_Ulink-SZ   (2024-09-29)


6*His-CDA

This part is composed of 6×His tag derived from a plasmid vector in the E. coli expression system and BBa_K5520000 derived from Bacillus pumilus strain. The 6×His tag is a commonly used protein tag consisting of six histidine (His) residues that is commonly used for CDA protein isolation and purification.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 85
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 544


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Categories
Parameters
None