Composite
Part:BBa_K5519004:Design
Designed by: Latifat Abdulsalam Group: iGEM24_KFUPM-Saudi-Arabia (2024-09-16)
AB_pTac_BborAMP1_TEVcut_GFP_His
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1093
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1093
Illegal NotI site found at 1235 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1093
Illegal BamHI site found at 1087
Illegal XhoI site found at 1244 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1093
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1093
Illegal NgoMIV site found at 1018
Illegal AgeI site found at 487 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since this construct is meant to be expressed in Escherichia coli, the sequence of BborAMP1 was codon optimized for expression in E.coli.
Source
BborAMP1 comes from the genome sequence of the thermophilic bacteria Brevibacillus Borstelensis AK1 APBN01000001.1