Regulatory

Part:BBa_K5515001:Design

Designed by: Chloe William   Group: iGEM24_McMasterU   (2024-10-02)


atpA 5' UTR and 16S Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1
    Illegal XhoI site found at 558
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Design required codon optimization for DNA synthesis as well as expression in algae.


Source

The sequence is derived from the genomic sequence of Escherichia coli

References