Composite
Part:BBa_K5507008:Experience
Designed by: josiazhang josiazhang Group: iGEM24_HW-ShanghaiUnited (2024-09-29)
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Applications of BBa_K5507008
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UNIQ7c1a7d21c589e001-partinfo-00000000-QINU UNIQ7c1a7d21c589e001-partinfo-00000001-QINU
a. Construction of BBa_K5507008 For the overexpression of the pgi gene from E. coli, the sacB locus, which encodes levansucrase, was selected as a homologous recombination site because knocking out this gene has no metabolic effects under glucose conditions. First, the kana expression cassette will replace the sacB gene locus as a selectable marker. Then, pgi will replace the kana gene, resulting in the loss of kana resistance, confirming successful gene insertion.