Terminator

Part:BBa_K5507001

Designed by: josiazhang josiazhang   Group: iGEM24_HW-ShanghaiUnited   (2024-09-29)


rrnB-T

The rrnB-T terminator is a transcriptional terminator derived from the ribosomal RNA operon of E. coli. It effectively halts transcription by forming a stable hairpin structure in the mRNA, followed by a string of uridine residues. This structure facilitates the release of the RNA polymerase from the DNA template, ensuring efficient termination of transcription. The rrnB-T terminator is widely used in molecular biology to enhance the yield and quality of recombinant RNA products, providing reliable termination in various expression systems.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 85


[edit]
Categories
Parameters
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