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Part:BBa_K5502000

Designed by: Chawanvit Tangwongsiri   Group: iGEM24_Institut-Le-Rosey   (2024-09-29)


A padlock probe used in rolling circle amplification for EGFR mutation (deletion on exon 19)

This is a biosensor for the EGFR mutation deletion of 23 bp from exon 19. The 3' and 5' arm of the padlock is complementary to the mutated sequence. It can be used in a rolling circle amplification process with the help of ligation using T4 ligase and replication using phi29 polymerase and random exonuclease-resistant primers. The middle sequence contains the flanking sequence of the left and right lettuce (BBa_K4245134 and BBa_K4245135), as the rolling circle product will be a binding site for the detection mechanism.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None