Part:BBa_K5499008
P2A is a self-cleaving peptide
P2A is a self-cleaving peptide derived from Porcine teschovirus-1, a short peptide (typically consisting of 18-22 amino acids) that mediates the cleavage of polyproteins during translation in eukaryotic cells. The P2A peptide is highly useful in gene therapy and protein expression because it can efficiently cleave multiple gene products into mature proteins without the need for proteases.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Profile
Name: P2A Peptide
Base Pairs:66 bp
Origins: The P2A peptide is derived from the Foot-and-Mouth Disease Virus (FMDV). It is a short, self-cleaving peptide that facilitates the co-expression of multiple proteins from a single mRNA transcript.
Properties: P2A enables a ribosomal skipping mechanism during translation, allowing for the production of distinct proteins without the need for additional cleavage enzymes. It is characterized by its efficiency in various expression systems, maintaining the integrity and functionality of the resulting proteins. The use of P2A is advantageous in applications requiring the simultaneous expression of multiple proteins, such as in vaccine development and gene therapy.
Usage and Biology
The P2A peptide is commonly used in synthetic biology for the co-expression of multiple proteins from a single open reading frame. By placing the P2A sequence between coding sequences, researchers can ensure that proteins are produced as separate entities despite being transcribed from the same gene. This technique is particularly useful in applications such as generating multivalent vaccines, producing fusion proteins, and engineering cells for therapeutic purposes.
P2A is a self-cleaving peptide derived from the Foot-and-Mouth Disease Virus (FMDV). It allows for the ribosomal skipping mechanism during translation, where the ribosome skips the peptide bond formation at the P2A site, leading to the production of distinct protein products. The unique feature of P2A is its ability to function efficiently at various expression levels without significantly affecting the protein folding or activity of the resulting proteins. This makes P2A an invaluable tool in protein engineering and molecular biology.
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