Part:BBa_K5499000
CMV enhancer
CMV enhancer is often combined with promoters like the CMV promoter or other constitutive promoters to increase transcriptional activity.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Profile
Name: CMV enhancer
Base Pairs: 304 bp
Origin: Derived from the Human Cytomegalovirus (HCMV), a widely used viral regulatory element in gene expression studies.
Properties: The CMV enhancer is a potent transcriptional activator element that significantly increases the transcription efficiency of associated promoters.
It is typically used in conjunction with the CMV promoter to achieve high levels of gene expression in mammalian cells.
Its enhancer effect is not cell-type specific, making it applicable to a wide range of mammalian cell lines. For this reason, it's extensively used in iGEM projects, as well as in biological research for expressing reporter genes, recombinant proteins, or in gene therapy studies.
Usage and Biology
CMV enhancer is widely used in mammalian expression vectors to drive high-level gene expression. It is often combined with promoters like the CMV promoter or other constitutive promoters to increase transcriptional activity. The CMV enhancer can enhance the expression of downstream genes, making it ideal for applications that require robust gene expression, such as protein production, gene therapy, and genetic research. It is also commonly used in viral vectors for gene delivery.
The CMV enhancer originates from the human cytomegalovirus (HCMV). It contains binding sites for several transcription factors, including NF-κB, CREB, and AP-1, which are involved in the regulation of immune responses and cell growth. The enhancer’s strong activity is due to its ability to recruit these transcription factors, which in turn increases the recruitment of RNA polymerase II to the promoter region. This robust activation leads to high levels of gene transcription in a variety of cell types, particularly in mammalian cells.
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