Composite
Part:BBa_K5498015
Designed by: Enyu LI Group: iGEM24_jky-wuhan-china (2024-09-30)
pfago
Recombinant vector pET-23a-PfAgo mainly consists of strong promoter T7, RBS(ribosomebinding site), PfAgo gene and T7 terminator. In order to facilitate the expression of purified PfAgo protein in E. coli, a 6×His tag was fused to the 5 'end of the PfAgo gene.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 816
Illegal EcoRI site found at 2406
Illegal XbaI site found at 47
Illegal PstI site found at 228 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 816
Illegal EcoRI site found at 2406
Illegal PstI site found at 228 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 816
Illegal EcoRI site found at 2406
Illegal BglII site found at 1472 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 816
Illegal EcoRI site found at 2406
Illegal XbaI site found at 47
Illegal PstI site found at 228 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 816
Illegal EcoRI site found at 2406
Illegal XbaI site found at 47
Illegal PstI site found at 228
Illegal AgeI site found at 2345 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 28
[edit]
Categories
Parameters
None |