DNA

Part:BBa_K5498010

Designed by: Enyu LI   Group: iGEM24_jky-wuhan-china   (2024-09-30)


MB:MB-EV71-2A

Our Molecular beacons contains loop, stem, quencher and fluorescent molecule. The loop contains a probe sequence that is complementary to gMB target sequence. And the stem is formed by the annealing of complementary arm sequences that are located on either side of the probe sequence. A fluorophore and a quencher is covalently linked to the end of the other arm. Molecular beacons do not fluoresce when they are free in solution.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


part2.png

[edit]
Categories
Parameters
None