Coding
Part:BBa_K5492203:Design
Designed by: Norbert Nyeste, Sandor Ban, Vojtech Vasut, Sara Simonka Group: iGEM24_TERMOSZ-Selye-HUN (2024-09-15)
Optimised DAO - pETDUET-1
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 129
Illegal XbaI site found at 47
Illegal PstI site found at 190 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 129
Illegal PstI site found at 190 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 129
Illegal BglII site found at 2392
Illegal BamHI site found at 123
Illegal BamHI site found at 2067
Illegal BamHI site found at 2321
Illegal XhoI site found at 937
Illegal XhoI site found at 2441 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 129
Illegal XbaI site found at 47
Illegal PstI site found at 190 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 129
Illegal XbaI site found at 47
Illegal PstI site found at 190
Illegal NgoMIV site found at 2411 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Restriction sites adapted to pETDUET-1, with adjustions also added for proper framing of the gene.
Source
The source of the part is its amino acid sequence which was retrieved from Uniprot (https://www.uniprot.org/uniprotkb/Q498N2/entry#sequences), and the work of iGEM20_SEHS-China BBa_K3684002 which provided the dna sequence we improved on.