Part:BBa_K5490033

pEgfp-C1

Vector for BBa_K5490024 insert

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 13
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal NheI site found at 3933
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BglII site found at 4681
  Illegal BamHI site found at 1
  Illegal XhoI site found at 4685
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 13
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal XbaI site found at 13
  Illegal NgoMIV site found at 583
  Illegal NgoMIV site found at 1866
  Illegal NgoMIV site found at 2149
  Illegal AgeI site found at 3942
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal BsaI.rc site found at 2361
  Illegal SapI.rc site found at 1715
  Illegal SapI.rc site found at 1925
  

We ordered an insert ( BBa_K5490024 ) containing a degradation signal derived from the pcDNA3.3_d2eGFP construct, a T2A peptide, and the luciferase gene with three target sequences. To facilitate directional cloning, we included two restriction sites: HindIII upstream and BamHI downstream. These allowed us to clone the insert into a plasmid already available in the lab.

The plasmid, pEGFPC1, contains a CMV promoter, an EGFP gene, and a multicloning site that includes both the HindIII and BamHI restriction sites, with a poly-A tail downstream.