Part:BBa_K5490030

IRES2

Viral RBS for mCherryNLS

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

IRES sequences are typically derived from viruses and can be inserted between two open reading frames (ORFs) in a single mRNA. This strategic placement allows for the translation of two proteins from the same polycistronic mRNA. IRES elements facilitate ribosome recruitment to the downstream ORF, enabling independent initiation of translation without requiring a 5' cap structure.

IRES sequences are widely applied in molecular biology, most notably as ribosome binding sites (RBS) in bicistronic or multicistronic constructs. They are especially useful when co-expressing multiple genes or proteins, such as in gene therapy, vaccine development, or studies requiring simultaneous expression of reporter and target genes.

Fitzgerald KD, Semler BL. Bridging IRES elements in mRNAs to the eukaryotic translation apparatus. Biochim Biophys Acta. 2009 Sep-Oct;1789(9-10):518-28. doi: 10.1016/j.bbagrm.2009.07.004. Epub 2009 Jul 23. PMID: 19631772; PMCID: PMC2783899.