Translational_Unit
CasRx

Part:BBa_K5490028:Design

Designed by: IOANNIS VASILEIOS ELAFROPOULOS   Group: iGEM24_IOANNINA   (2024-09-25)


CasRx


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 499
    Illegal EcoRI site found at 919
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 499
    Illegal EcoRI site found at 919
    Illegal NotI site found at 2908
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 499
    Illegal EcoRI site found at 919
    Illegal BamHI site found at 2899
    Illegal XhoI site found at 2374
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 499
    Illegal EcoRI site found at 919
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 499
    Illegal EcoRI site found at 919
    Illegal NgoMIV site found at 187
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

none


Source

Source:Silvana Konermann Laboratory of Molecular and Cell Biology, Salk Institute for Biological Studies, 10010 N Torrey Pines Rd, La Jolla, CA 92037, USA Direct repeat sequence,firstly identified at Ruminococcus

References