Measurement
RcnA/RcnR

Part:BBa_K549001:Design

Designed by: Jara Radeck   Group: iGEM11_LMU-Munich   (2011-08-26)

Ni(II) dependent rcnA-promoter and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 863


Design Notes

This part should be expressed in E. coli, in order to use the rcnA/rcnR-System. The regulator rcnR is naturally expressed in E.coli and has not to be added to the cells.


Source

GFP is from E0040, prcnA is from genomic DNA (E. coli K12)