Part:BBa_K5487125

It contains the gene sequence encoding the LCI-M3 anchor peptide, a TEV cleavage site, a His-tag, a CG-linker, and sfGFP. We chose pET30A as the vector because it contains multiple T7 promoters and terminators, as well as kanamycin resistance. In this design, we introduced a GC-linker to ensure that the two components (sfGFP and the anchor peptide) can function as independent modules, while also enhancing their expression levels. Additionally, because the relatively large molecular weight of sfGFP may interfere with subsequent gold nanoparticle modifications compared to the anchor peptide, we inserted a TEV protease cleavage site and a His-tag between the two to facilitate subsequent purification of the anchor peptide using TEV protease cleavage followed by nickel column purification.