Coding

Part:BBa_K5487113:Design

Designed by: Xuerui Tao   Group: iGEM24_UESTC-China   (2024-09-03)


Rep101-pSC101 ori-rrnB T1 terminator Backbone


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1277
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1277
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 32
    Illegal XhoI site found at 13
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1277
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1277
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Obtained after synthesizing plasmid PHCY163 at Bio, transformed and amplified within E. coli DH5α


Source

Rep101 and pSC101 come from Plasmid PSC101, which is temperature sensitive in E.coli.

References

Huang, C., Guo, L., Wang, J. et al. Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering. Appl Microbiol Biotechnol 104, 7943–7956 (2020). https://doi.org/10.1007/s00253-020-10819-1