Coding

Part:BBa_K5487102:Design

Designed by: wenzhuo yang   Group: iGEM24_UESTC-China   (2024-09-02)


Cg-Def-v2 S19K/K10L/N13H


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The vector in which it participates in the construction should include sfGFP,The main reason for co-expressing sfGFP with the anchor peptide is that the anchor peptides have a small molecular weight, making it difficult to verify their expression using conventional methods such as protein concentration assays.

Source

a first PLABS high-throughput screening platform to design and evaluate the protein engineering of material-specific binding peptides has been developed and validated through a KnowVolution campaign. As a result, a Cg-Def variant V2 with significantly improved PLA binding specificity over PP was yielded

References

Lu, Y.; Hintzen, K. W.; Kurkina, T.; Ji, Y.; Schwaneberg, U., A Competitive High-Throughput Screening Platform for Designing Polylactic Acid-Specific Binding Peptides. Adv Sci (Weinh) 2023, 10 (29), e2303195.