Part:BBa_K5487004
T7 Promoter+lac operator+RBS+TphA2+RBS+TphA1+Term+T7 promoter+lac operator+RBS+TphA3+RBS+TphB2+Term
TphA1, TphA2, TphA3 form the terephthalate 1,2-dioxygenase enzyme complex, which is pivotal in the biodegradation of PET monomers, notably terephthalic acid (TPA). These proteins orchestrate the oxidative cleavage of the aromatic ring's carbon-carbon bonds within TPA, initiating the metabolic cascade that upgrades PET waste into valuable chemical intermediates and products.
TphA1 is a key catalytic subunit within the terephthalate 1,2-dioxygenase complex, responsible for the initial binding and oxidative attack on TPA. Characterized by the presence of a non-heme iron center, this enzyme is essential for the ring-opening of the aromatic structure, which represents the first step in the degradation of PET. TphA2 functions in concert with TphA1, likely participating in the electron transfer processes that are critical to the dioxygenase activity. It supports the catalytic reaction by maintaining the structural integrity of the enzyme's active site and is involved in the reduction of molecular oxygen to water, a byproduct of the oxidative mechanism. TphA3, an integral subunit of the terephthalate 1,2-dioxygenase complex, contributes to the electron transport chain, potentially facilitating the transfer of electrons from the active site to other components of the complex or to external electron carriers. This role is crucial for the overall catalytic efficiency of the enzyme. TphB2（1,4-Dicarboxylic Acid Dehydrogenase )，subsequent to the initial enzymatic action, transforms the intermediate dihydroxy-3,5-cyclohexadiene-1,4-dicarboxylic acid (DHCD) into protocatechuate acid(PCA).
In summary, the synergistic operation of these enzymes within a metabolic pathway in a host organism streamlines the conversion of PET-derived monomers into beneficial chemicals. This biochemical process aimed at crafting sustainable recycling methods for PET waste and harnessing renewable resources to produce precious chemicals.
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2326
Illegal NheI site found at 2391
Illegal NheI site found at 4055 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1457
Illegal NgoMIV site found at 1766
Illegal NgoMIV site found at 3291 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 3163
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