Regulatory
Part:BBa_K5486000:Design
Designed by: Julian Borbeck Group: iGEM24_Tuebingen (2024-10-01)
EL222-YdfI fusion regulator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 81
Illegal AgeI site found at 306 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Optimal rewiring point to fuse the domains was determined using ClusterControl
Source
Backtranslation from protein sequence using codon optimization for E.coli