Regulatory

Part:BBa_K5486000:Design

Designed by: Julian Borbeck   Group: iGEM24_Tuebingen   (2024-10-01)


EL222-YdfI fusion regulator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 81
    Illegal AgeI site found at 306
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimal rewiring point to fuse the domains was determined using ClusterControl


Source

Backtranslation from protein sequence using codon optimization for E.coli

References