Composite
Part:BBa_K5477030:Design
Designed by: Isaiah Kate Malana Escobar Group: iGEM24_UCopenhagen (2024-09-26)
XRE-pMEL1-NanoLuc reporter module
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 59
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 59
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 59
Illegal BamHI site found at 3 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 59
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 59
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The pUUS plasmid is a multi-copy plasmid used in yeast, carrying a URA3 marker for selection in uracil auxotrophic strains. In our system, pUUS is utilized in the reporter module, enabling the expression of the reporter gene, NanoLuc.
Source
Homo sapiens and Saccharomyces cerevisiae